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KMID : 0357119930150010083
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Korean Journal of Immunology
1993 Volume.15 No. 1 p.83 ~ p.89
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Nitric Oxide Production of RAW 264.7 Cell Line by the Stimulation of Cytokines and Lipopolysaccharide
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Kim Young-Deog
Jun Chang-Duk
Lee Byung-Soon
Lee Bok-Soo
Park Seok-Don
Chung Hun-Taeg
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Abstract
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Macrophages have been implicated as a major clas of effector in the host response to neoplasia. Cells of the monlocyte-macrophage lineage are known to exhibit tumoricidal activity following stimulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopolysaccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect.
But much of what is known about the inductijon and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals.
In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells shlowed above 99% positive of pan macrophage marker by immunogistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I : C. Incubation of RAW 264.7 cells with-γIFN for 48 hours in the presence of LPS agumednted NO release in a dose dependent manner. Whereas, treatment of anti-TNE-αantibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO? by γ-IFN plus LPS activated macrophages. The production of NO was aso inhibiedd reversibly or irreversibly by NGMMA, NAA, arginase or DPI.
Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.
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KEYWORD
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Reactive Nitrogen Intermediate(RNI)
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